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. Author manuscript; available in PMC: 2021 Dec 30.
Published in final edited form as: Nat Chem Biol. 2021 Jul 8;17(8):896–905. doi: 10.1038/s41589-021-00823-5

Fig. 5: K29-linked polyubiquitination is involved in cell cycle regulation.

Fig. 5:

Gel panels in this figure are representative of two independent experiments; n = 2. a, HeLa cells were transfected with Tra, Tra-CIM or an empty vector for 24-27 h. The cell lysates were analyzed by Western blot using a c-myc antibody and sAB-K29, respectively. b, Cell cycle analysis of HeLa cells transfected with Tra or Tra-CIM for 24-27 h using flow cytometry. Representative flow cytometry graphs from three biological replicates are shown (a total of 3 injections). Highlighted areas were subjected to cell cycle analysis. c, Quantification of the cell cycle analysis results in panel b. (n = 3 biological replicates, mean ± SD, two-sided Student’s t-test).