Fig 3. Matrix Metalloprotease (MMP)-12 is chronically upregulated in alveolar and interstitial macrophages (MΦ) by Ascaris.

(A-C) mRNA level of (A) MMP12, (B) MMP9, and (C) neutrophil elastase (ELANE) from Ascaris infected and naïve mice post infection. (D-F) Flow cytometry analysis on F4/80⁺ CD64⁺ CD11b^- SigletF⁺ Alveolar macrophages and F4/80⁺ CD64⁺ CD11b⁺ interstitial macrophages (D) Representative flow plot of MMP12 on alveolar macrophages and the aggregate data of MMP12^high alveolar macrophages expressed as percentages and absolute cell numbers and representative histograms and median fluorescence intensity (MFI) of MMP12 data are shown. (E) Representative flow plot of MMP12 on interstitial macrophages and the aggregate data of MMP12^high interstitial macrophages expressed as percentages and absolute cell numbers and representative histograms and median fluorescence intensity (MFI) of MMP12 data are shown. (n≥3, mean ± S.E.M, n.s.: not significant, *p<0.05, **p<0.01, ***p<0.001, using two tailed Student’s t-test. Data are representative of two independent experiments)