Figure 2.

Suppression of myogenesis in PAK4‐overexpressing satellite cells (SCs). (A–C) Immunofluorescence staining and western blotting analysis for PAK4 in SCs cultured in growth medium for different time periods (from G₀ to G₄) followed by 4 day culture in differentiation medium (D4). (D, E) SCs were infected with PAK4 and mutant PAK4 adenoviruses and cultured in growth medium (GM) for 6 h and in differentiation medium (DM) for another 4 days. Protein levels of myogenic markers (MyHC and Myog) and myotube formation were determined. Values are mean ± SD. ^** P < 0.01 vs. Ad‐LacZ; ^## P < 0.01 vs. Ad‐PAK4.