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. 2021 Aug 25;12(6):1741–1756. doi: 10.1002/jcsm.12770

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© 2021 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Pim1 knockout alleviated intramuscular adipose tissue infiltration in aging mice. (A) Fat mass measured through dual energy x‐ray analysis (%). (B) Oil red staining, immunofluorescent staining of perilipin 1, and immunohistochemical staining of adiponectin (scale = 50 μm). (C) Adipose tissue content (%). (D) Immunofluorescent analysis of perilipin 1 expression. (E) Immunohistochemical analysis of adiponectin expression. (F) Detection of adiponectin expression through western blotting; GAPDH: internal reference. (G) Relative protein‐expression level of adiponectin. N = 6–8; *P < 0.05, **P < 0.01, ***P < 0.001.