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. 2021 Aug 25;12(6):1741–1756. doi: 10.1002/jcsm.12770

Figure 6.

Figure 6

Senescent PDGFRα+ mesenchymal progenitors had increased adipogenic potency. (A) Oil red staining of young and senescent PDGFRα+ mesenchymal progenitors after 0 day, 3 days, 6 days, or 9 days adipogenic induction (scale = 50 μm). (B) Analysis of OD510. (C) Detection of adiponectin, PPARγ, C/EBPβ, C/EBPδ, and FABP4 expression through western blotting; GAPDH: internal reference. (D–H) Relative protein‐expression levels of adiponectin, PPARγ, C/EBPβ, C/EBPδ, and FABP4. (I) Detection of Pim1 expression through western blotting; GAPDH: internal reference. (J) Relative protein‐expression level of Pim1. N = 3–4; *P < 0.05, **P < 0.01, ***P < 0.001.