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. 2021 Aug 25;12(6):1741–1756. doi: 10.1002/jcsm.12770

Figure 7.

Figure 7

Pim1 inhibitor SGI1776 significantly inhibited the adipogenic differentiation of senescent PDGFRα+ mesenchymal progenitors. (A) Oil red staining of young and senescent PDGFRα+ mesenchymal progenitors after 6 days adipogenic induction with different concentrations of SGI1776 (scale = 50 μm). (B) Analysis of OD510. (C) Detection of adiponectin expression through western blotting; GAPDH: internal reference. (D) Relative protein‐expression level of adiponectin. (E) Adiponectin content in supernatant. (F) Senescence associated β‐galactosidase staining (scale = 100 μm). (G) Content of SA‐β‐GAL+ cells (%). (H) Detection of GLB1, p53, and p16 expression through western blotting; GAPDH: internal reference. (I–K) Relative protein‐expression levels of GLB1, p53, and p16. (L) Edu staining of young and senescent PDGFRα+ mesenchymal progenitors (scale = 100 μm). (M) Content of Edu+ cells (%). N = 3–4; *P < 0.05, **P < 0.01, ***P < 0.001.