FIGURE 6.

The effect of G4 stabilizing ligands on gene expression. (A) Schematic illustration of PQS-recombinant plasmids construction. Plasmids containing empty pEGFP-N1 vector (B), D117L (C), or P1192R (D) were transfected into 293T cells in the presence or absence of 20 μM of each ligand. After 24 h of incubation, the expression of EGFP in each well was assessed by confocal microscopy. (E) Quantitative fluorescence signal using corrected total cell fluorescence method for EGFP in cells transfected with plasmids containing D117L, P1192R, and the empty vector. ns, not significant; ***p < 0.001.