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Cytotoxicity and antiviral activity of NMM against ASFV in cell culture. (A) Cytotoxicity of NMM in PAM cells was determined by MTT Assay. (B) Antiviral activity of NMM against ASFV. PAM cells were infected with ASFV strain Wuhan 2019-2 at an MOI of 0.1 in the presence of various doses of NMM (0, 0.03, 0.1, 0.3, 1, 3, and 10 μM). 72 h post-infection, viral yield in the culture supernatant was quantified by qRT-PCR.
