| Antibody |
BUV395 anti-CD11b (rat monoclonal) |
BD Biosciences |
Clone M1/70 |
Flow cytometry (1:200) |
| Antibody |
Biotin anti-F4/80(rat monoclonal) |
BioLegend |
Clone BM8 |
Flow cytometry(1:200) |
| Antibody |
FITC anti- I-A/I-E (MHC-II) (rat monoclonal) |
BD Biosciences |
Clone 2G9 |
Flow cytometry (1:200) |
| Antibody |
BB515 anti-Siglec-F (rabbit monoclonal) |
BD Biosciences |
CloneE50-2440 |
Flow cytometry(1:200) |
| Antibody |
PE anti-CD11c(hamster monoclonal) |
BD Biosciences |
Clone N418 |
Flow cytometry(1:200) |
| Antibody |
PerCP-Cy5.5 anti-Ly6G(rat monoclonal) |
BioLegend |
Clone IA8 |
Flow cytometry(1:200) |
| Antibody |
BUV737 anti-B220(rat monoclonal) |
BD Biosciences |
CloneRA3-6B2 |
Flow cytometry (1:300) |
| Antibody |
PE-Cy7 anti-Ly6C(rat monoclonal) |
BD Biosciences |
Clone AL-21 |
Flow cytometry(1:200) |
| Antibody |
APC-Cy7 anti- TCRb(hamster monoclonal) |
BD Biosciences |
CloneH57-597 |
Flow cytometry(1:300) |
| Antibody |
Anti-CD16/CD32 (rat monoclonal Fc block) |
BD Biosciences |
Clone 2.4G2 |
Flow cytometry(1:200) |
| Peptide, recombinant protein |
Streptavidin |
BD Biosciences |
BV421-streptavidin |
Flow cytometry (1:400) |
| Chemical compound, drug |
Zombie Aqua |
BioLegend |
Cat#: 423101 |
Flow cytometry(1:700) |
| Chemical compound, drug |
AF647-ZOL |
BioVinc, CA |
Cat#: SKU BV501001
|
Flow cytometry(47.5 μg/40 nmoles per mouse i.v. dose) |
| Chemical compound, drug |
Zoledronic acid |
Sigma-Aldrich |
Cat#: SML0223 |
500 μg/kgi.v. dose |
| Chemical compound, drug |
LPS (Escherichia coli) |
Sigma-Aldrich |
O111:B4 |
10 μg i.n. or 100 μg i.p. per mouse |
| Chemical compound, drug |
Biotin-GPP |
doi.org.10.1080/21541248.2015.1085485
|
|
Prof Kirill Alexandrov |
| Peptide, recombinant protein |
REP-1(zebrafish) |
doi.org.10.1080/21541248.2015.1085485
|
|
Prof Kirill Alexandrov |
| Peptide, recombinant protein |
GGTase-II(rat) |
doi.org.10.1080/21541248.2015.1085485
|
|
Prof Kirill Alexandrov |
| Peptide, recombinant protein |
Streptavidin-680RD |
LiCOR |
P/N: 925-68079 |
Western blotting (1:20,000) |
| Antibody |
Anti-IL-1β(goat polyclonal) |
R&D Systems |
Cat#:AF-401-NA |
Western blotting (1:1000) |
| Antibody |
HRP anti-goat IgG(donkey polyclonal) |
Thermo Fisher |
Cat#:A15999 |
Western blotting (1:5000) |
| Commercial assay or kit |
SuperSignal West Pico substrate |
Thermo Fisher |
Cat#: 34580 |
Western blotting |
| Commercial assay or kit |
Bio-Plex immunoassay kit |
Bio-Rad |
Cat#: M60009RDPD |
Cytokine and chemokine assay |
| Recombinant protein |
M-CSF (human) |
Sino Biological |
Cat#: 11792-HNAH |
Cell culture supplement (50 ng/mL) |
| Chemical compound |
Nigericin |
Sigma-Aldrich |
Cat#: N7143 |
|
| Chemical compound |
IPP triammonium salt |
Sigma-Aldrich |
Cat#: I0503-1VL |
LC-MS/MS standard |
| Chemical compound |
DMAPP triammonium salt |
Toronto Research Chemicals |
Cat#: 63180-1MG |
LC-MS/MS standard |
| Software, algorithm |
FlowJo software |
Becton Dickinson |
RRID:SCR_008520
|
Version 10.6.2 |
| Software, algorithm |
MassHunter Quantitative Analysis software |
Agilent |
|
Version B08.00.00 |
