| Gene (Homo sapiens) |
ATP6V0A1 |
NA |
Q93050 (VPP1_HUMAN) |
V-type proton ATPase 116 kDa subunit a1 |
| Gene (Homo sapiens) |
ATP6V0A2 |
NA |
Q9Y487 (VPP2_HUMAN) |
V-type proton ATPase 116 kDa subunit a2 |
| Cell line (Homo sapiens) |
Lenti-X 293T Cell Line |
Clontech |
Cat#632180 |
|
| Cell line (Homo sapiens) |
HUVEC |
Lonza |
Cat#C2519A |
|
| Antibody |
anti-VE-Cadherin (mouse monoclonal) |
R&D Systems |
Cat#123413RRID:AB_2260374
|
(1:1,000) |
| Antibody |
anti-ZO-1 (rabbit monoclonal) |
CST |
Clone:D6L1ERRID:AB_2798287
|
(1:50) |
| Antibody |
anti-VWF (rabbit polyclonal) |
Dako/Agilent Technologies |
Cat#P0226RRID:AB_579516
|
(1:1,000) |
| Antibody |
anti-VWF (sheep polyclonal) |
Abcam |
Cat#ab11713RRID:AB_298501
|
(1:500) |
| Antibody |
anti-V0c (rabbit polyclonal) |
Thermo-Fisher Scientific |
Cat#PA5-66746RRID:AB_2662731
|
(1:25) |
| Antibody |
anti-V1A (rabbit polyclonal) |
Thermo-Fisher Scientific |
Cat#PA5-65137RRID:AB_2662833
|
(1:125) |
| Antibody |
anti-V0a1 (rabbit polyclonal) |
Novus Biologicals |
Cat#NBP1-89342RRID:AB_11015740
|
(1:200) |
| Antibody |
anti-HA (mouse monoclonal) |
Covance |
Clone:16B12RRID:AB_10064068
|
(1:1,000) |
| Antibody |
anti-TGN46 (sheep polyclonal) |
Bio-Rad |
Cat#AHP500GTRRID:AB_2203291
|
(1:500) |
| Antibody |
anti-V0a2 (rabbit polyclonal) |
Atlas Antibodies |
Cat#HPA044279RRID:AB_10961274
|
(1:50) |
| Antibody |
anti-V0a2 (rabbit polyclonal) |
My BioSource |
Cat#MBS8527160 |
(1:100) |
| Antibody |
anti-α-tubulin (mouse monoclonal) |
CST |
Clone:DM1ARRID:AB_1904178
|
(1:2,000) |
| Antibody |
anti-PKD2 (rabbit monoclonal) |
Abcam |
Clone:EP1495YRRID:AB_922233
|
(1:200) |
| Antibody |
anti-PKD2 (rabbit polyclonal) |
GeneTex |
Cat#C1C3RRID:AB_1951437
|
(1:200) |
| Antibody |
anti-phospho-PKD/PKCμ(Ser744/448) (rabbit polyclonal) |
CST |
Cat#2054RRID:AB_2172539
|
(1:1,000) |
| Antibody |
anti-PKD/PKCμ (rabbit monoclonal) |
CST |
Clone:D4J1NRRID:AB_2800149
|
(1:1,000) |
| Antibody |
Alexa 488-, 555-, 647 secondaries |
Thermo Fisher Scientific |
|
(1:400) |
| Antibody |
Cy3-secondary |
Jackson Immunoresearch |
|
(1:400) |
| Recombinant DNA reagent |
pLVSIN-CMV Pur Vector |
Clontech |
Cat#6183 |
|
| Recombinant DNA reagent |
Mission shRNA plasmid non-targeting control |
Sigma-Aldrich |
SHC016 |
|
| Recombinant DNA reagent |
Mission shRNA plasmid against V0a1 |
Sigma-Aldrich |
TRCN0000380234 |
Mainly used throughout the study. |
| Recombinant DNA reagent |
Mission shRNA plasmid against V0a1 |
Sigma-Aldrich |
TRCN0000333635 |
Used in Figure 6—figure supplement 2B. |
| Recombinant DNA reagent |
Mission shRNA plasmid against V0a2 |
Sigma-Aldrich |
TRCN0000043494 |
|
| Chemical compound, drug |
Bafilomycin A1 |
Cayman Chemicals |
Cat#11038 |
100 nM |
| Chemical compound, drug |
iFluor555-conjugated phalloidin |
Cayman Chemicals |
Cat#20552 |
(1:400) |
| Chemical compound, drug |
Concanamycin A |
AdipoGen Life Sciences |
Cat#BVT-0237 |
100 nM |
| Chemical compound, drug |
Brefeldin A |
Thermo Fisher Scientific |
Cat#B7450 |
100 ng/ml |
| Chemical compound, drug |
Histamine |
Sigma-Aldrich |
Cat#H7250 |
100 μM |
| Chemical compound, drug |
TransIT Lenti transfection reagent |
Clontech |
Cat#MIR6600 |
|
| Chemical compound, drug |
Lentiviral High Titer Packaging Mix |
Clontech |
Cat#6194 |
|
