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. 2021 Apr 27;13:17590914211010647. doi: 10.1177/17590914211010647

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — HOTAIR Overexpression Reversed the Inhibitory Effect of PAN on Neuronal Ferroptosis. (A) The expression of HOTAIR in neuronal cells was measured by qRT-PCR. (B) HT22 cells or cortical neurons were transfected with pcDNA3.1-HOTAIR. The expression of HOTAIR in the HT22 cells was measured by qRT-PCR. (C) The viability of neuronal cells was assessed by MTT assay. (D) The level of MDA in neuronal cells was measured by a Lipid Peroxidation Assay Kit. (E) The lipid ROS level was detected by flow cytometry. (F) The GSH level in neuronal cells was detected by a Glutathione Assay Kit. (G) The Fe²⁺ content of neuronal cells was tested by an Iron Assay Kit. (H) ACSL4, SLC7A11 and GPX4 expression in neuronal cells was tested by western blot. β-actin was used for normalization. ^*P<0.05, ^**P<0.01, ^***P<0.001. (n = 3 in every group). One-way analysis of variance (ANOVA) and Student’s t-test were performed to analyse the data.