Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 27;13:17590914211010647. doi: 10.1177/17590914211010647

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 4. — PAN Alleviated ACSL4-Dependent Neuronal Ferroptosis. (A) ACSL4 mRNA levels in neuronal cells were measured by qRT-PCR. (B) ACSL4 expression in neurons was tested by western blot. β-actin was used for normalization. Neuronal cells were transfected with the pcDNA3.1-ACSL4 overexpression vector. Then, (C) ACSL4 mRNA levels in neuronal cells were assessed by qRT-PCR. (D) Neuronal cell viability was tested by MTT assay. (E) The MDA level in neuronal cells was tested by a Lipid Peroxidation Assay Kit. (F) The lipid ROS level was detected by flow cytometry. (G) The GSH level was measured by a Glutathione Assay Kit. (H) The Fe²⁺ content of neurons was assessed by an Iron Assay Kit. (I) ACSL4, SLC7A11 and GPX4 expression was measured by western blot. β-actin was used for normalization. ^*P<0.05, ^**P<0.01, ^***P<0.001. (n = 3 in every group). One-way analysis of variance (ANOVA) and Student’s t-test were performed to analyse the data.