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. 2022 Jan 4;132(1):e146658. doi: 10.1172/JCI146658

Figure 4. Effects of NAM treatment on transplanted corneal endothelial precursors.

Figure 4

(A) Expressions of neural crest and corneal endothelial genes in the pretransplanted (Pre) and transplanted cells at day 7 and day 14 after transplantation by qPCR using human-specific primers. n = 4. *P < 0.05, **P < 0.01 versus the pretransplanted CEPs. (B) Double staining of neural crest markers P75, AP-2β, and corneal endothelial markers ZO-1, ATP1A1, and SLC4A11 at day 14 after transplantation. Cells from human origin were stained with HuNu. Nuclei were stained with DAPI. Scale bar: 50 μm. (C) Expressions of EnMT and senescence-associated genes at day 7 after transplantation by qPCR. n = 3, **P < 0.01. (D) Double staining of α-SMA and fibronectin, and SA-β-gal staining at day 7 after transplantation. The transplanted cells of human origin were stained by HuNu. Nuclei were stained with DAPI. Scale bar: 50 μm. One-way ANOVA with Tukey’s HSD test (A) and 2-tailed Student’s t test (C).