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. 2022 Jan 4;132(1):e150051. doi: 10.1172/JCI150051

Figure 1. Low level of SIRT6 expression was associated with increased risk of vascular calcification.

Figure 1

(A) The qPCR showed expression of SIRT1-7 in WT VSMCs with different calcification statuses. SIRT4 was not detected in VSMCs (n = 4 per group). Data were expressed as mean ± SD, *P < 0.05. (B) SIRT6 mRNA levels in PBMCs from patients with CKD with (n = 27) or without (n = 12) VC. Data were expressed as mean ± SD. (C) Correlation between the SIRT6 mRNA level and VC scores in patients with CKD (n = 39, the Pearson’s correlation coefficient R value and the P value are shown). (D) von Kossa assay and IF staining for SIRT6 in radial arteries sections from hemodialysis patients with CKD (n = 4 per group). Scale bars: von Kossa 100 μm; IF 50 μm. (E) The bars showing SIRT6 protein expression (mean ± SD; n = 4 per group; AU) in nuclei of aortic tissues between patients with CKD with and without VC. Statistical significance was assessed using 1-way ANOVA followed by Dunnett’s test (A) and 2-tailed t tests (B and E).