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. 2022 Jan 4;132(1):e150051. doi: 10.1172/JCI150051

Figure 2. SIRT6 attenuated VC.

Figure 2

(A) Computed tomography (CT) images showing calcification in the abdominal aorta. The green arrows and circle indicated the calcification in abdominal aorta of the WT mouse (n = 12 per group). The bar chart shows the relative VC Agatston score (fold change) of mouse aortas. Scale bars: 10 mm. (B) Representative von Kossa staining of abdominal aorta sections (n = 12 per group). Scale bars: 100 μm. (C) Western blot shows SIRT6 protein in abdominal aorta was reduced in VC. (D and E) VSMCs were exposed to Pi (3.0 mM) for 7 days and then stained for mineralization by Alizarin red (D), and the quantitative analysis of calcium content (E) and ALP (F) were detected (n = 3 per group). (G) SIRT6 protein expression was reduced in WT and SIRT6-Tg VSMCs in response to Pi (3.0 mM) treatment (n = 4 per group). (H–J) WT and SIRT6-Tg VSMCs were pretransfected with siSIRT6 or si-negative control (siNC) and then exposed to Pi (3.0 mM) for 7 days. VSMCs were stained for mineralization by Alizarin red S (H), and calcium content (I) and ALP (J) were quantified (n = 3 per group). Statistical significance was assessed using 1-way ANOVA followed by Dunnett’s test (A, C–F, I, and J). *P < 0.05. All values are mean ± SD.