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. 2022 Jan 4;132(1):e151268. doi: 10.1172/JCI151268

Figure 5. GSDMD is essential for recruitment of neutrophils/monocytes to the I/R heart.

Figure 5

(A–F) Flow cytometric analysis and quantification of Cd11b+Ly6G+ neutrophils and Cd11b+Ly6C+ monocytes in heart (A and B), blood (C and D), or bone marrow (BM) (E and F) from WT or Gsdmd−/− mice at different reperfusion time points (3 hours, 6 hours, 12 hours, 24 hours) after I/R or sham surgery. Corresponding n values are indicated in the plots. The statistical significance of sham versus 3, 6, 12, or 24 hours is indicated (n = 3–5). (G and H) Flow cytometric analysis (G) and quantification (H) of Cd11b+Ly6G+ neutrophils and Cd11b+Ly6C+ monocytes in heart (left), blood (middle), or BM (right) from WT or Gsdmd−/− mice 24 hours after I/R (n = 3). Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by 1-way ANOVA followed by Bonferroni’s multiple-comparison test (B, D, and F) or multiple 2-tailed Student’s t test (H). NS, not significant.