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Duplex PCR strategy. Amplified DNA fragments from M. bovis (lane 1), M. avium subsp. (lane 2), and M. avium subsp. paratuberculosis (lane 3) were separated by electrophoresis in a 2% agarose gel. The lengths (in base pairs) of the amplified fragments, as well as of the BglI- and HinfI-cleaved pBR328 DNA fragments (molecular weight marker) (lane 4), are indicated on the left- and righthand sides of the gel, respectively.
