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. 2021 Dec 23;2021:4125432. doi: 10.1155/2021/4125432

Figure 3.

Figure 3

LINC01320 targeting miR-324-3p. (a) starBase and LncBase jointly predicted the target gene of LINC01320. (b) Expression of miR-324-3p and miR-496 in GSE59856 chip. (c) qRT-PCR was used to detect the relative expression of miR-324-3p and miR-496 in pancreatic cancer tissues. (d) Pearson test was used to analyze the correlation between LINC01320 and miR-324-3p expression in tumor tissues of pancreatic cancer patients. (e) Targeted binding site of LINC01320 and miR-324-3p. (f) Double luciferase report confirmed that LINC01320 targeted miR-324-3p. (g) RIP experiment confirmed that both LINC01320 and miR-324-3p were precipitated by Ago2 protein. (h) qRT-PCR was used to detect the miR-324-3p relative expression in pancreatic cancer cells transfected with LINC01320#1. ∗P < 0.05,∗∗P < 0.01,∗∗∗P < 0.001.