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. 2021 Dec 17;12:730970. doi: 10.3389/fimmu.2021.730970

Figure 2.

Figure 2

NK cell functions are altered in leukemic mice. Cells were freshly extracted from the spleen of leukemic or control mice pre-injected or not with 100μg Poly(I:C) 16h prior to sacrifice. Cells were then stained immediately (C), or after four hours of culture alone, or with YAC-1/FLB1/C1498 cells, or with PMA and ionomycin, in the presence of Golgi-stop, Golgi-plug and anti-CD107a antibody (A, B, D). Cells were then analyzed by flow cytometry. (A) Zebra plots show the level of CD107a exposure and IFN-γ production by total NK cells primed with Poly(I:C) and cultured with YAC-1 cells. (B) The graphs show the percentages of NK cell IFN-γ production and degranulation (CD107a exposure) in result to all stimulations (n=9-12 mice/group in at least three independent experiments, except for YAC-1: n=3 mice/group in one experiment). (C) The level of perforin and granzyme B production, as measured by MFI, by total NK cells after priming with Poly(I:C) (n=7-8 mice/group in two independent experiments). (D) The percentages of IFN-γ production and degranulation by NK cells primed with Poly(I:C) and cultured with FLB1 or C1498 AML cell lines (n=6-11 mice/group in at least two independent experiments). The values are presented as the mean +/- SEM. *p < 0.05, **p < 0.01, ***p <0 .001, NS, Non Significant, as determined by Mann-Whitney test.