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. 2021 Dec 17;12:768198. doi: 10.3389/fimmu.2021.768198

Figure 3.

Figure 3

Overexpression of C3a in reactive astrocytes reduces HI-induced neurodegeneration, astrocyte activation, and microglia proliferation. (A) Representative images of ipsilesional CA3 stained with FJC and antibodies against NeuN, GFAP and Iba-1 3 weeks after HI induction. Scale bar = 50 µm. (B–D) Number of FJC+ cells (B) and NeuN+ cells (C), relative GFAP+ area (D), and density of Iba-1+ cells (E) in CA3 of WT and C3a/GFAP mice. IL, ipsilesional; CL, contralesional. **P < 0.005, ****P < 0.0005 vs WT; #### P < 0.0005 vs contralesional. Data were analyzed by two-tailed unpaired t test (B) or two-way ANOVA and Tukey’s posthoc analysis (C–E). Numbers in bars (B) are number of positive mice/total number of mice. (C–E) n = 7–9 (WT) and 11–14 (C3a/GFAP). Values are mean ± SEM.