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. 2021 Dec 17;12:768198. doi: 10.3389/fimmu.2021.768198

Figure 5.

Figure 5

Intranasal C3a treatment reduces HI-induced neurodegeneration and reactive astrogliosis in WT mice treated with PBS or C3a. (A) Relative expression of C3aR mRNA after HI. n = 3. (B) In the ipsilesional CA3 3 days after HI, C3aR is expressed predominantly by Iba-1+ microglia. Scale bar = 50 µm. (C) Representative images of ipsilesional CA3 stained with FJC and antibodies against NeuN, GFAP and Iba-1 7 weeks after HI induction. Scale bar = 50 µm. (D–G) Number of FJC+ cells (D), number of NeuN+ cells (E), relative GFAP+ area (F), and (G) density of Iba-1+ cells in CA3. IL, ipsilesional; CL, contralesional. *P < 0.05, **P < 0.005 vs PBS; # P < 0.05, ### P < 0.001, #### P < 0.0005 vs contralesional. Data were analyzed by two-tailed unpaired t test (D) or two-way ANOVA and Tukey’s posthoc test (E–G). Numbers in bars (D) indicate number of positive mice/total number of mice. (E–G) n = 13–14 (PBS) and 9–15 (C3a). Values are mean ± SEM.