FIGURE 8.

ITGB1 expression modulated HCC tumor growth in vivo. (A) Gating strategy for isolating ITGB1^high and ITGB1^lowcells from HCC cells. (B) Representative images of subcutaneous tumors formed by ITGB1^low or ITGB1^high HCC1 cells in mice. Scale bar = 1 cm. (C) The quantification of tumor volume of subcutaneous tumors of HCC cells (n = 5 per group). (D) Representative confocal images of PXN in tissues from ITGB1^high and ITGB1^low HCC1 cell-formed tumors. Scale bar = 100 µm. (E) The quantification of the protein expression of PXN by immunostaining analysis. Samples were probed by anti-PXN (red) Ab and counterstained by DAPI (blue). (F) Representative confocal images of YWHAZ in tissues from ITGB1^high and ITGB1^low HCC1 cell-formed tumors. Scale bar = 100 µm. (G) The quantification of protein expression of YWHAZ by immunostaining analysis. Samples were probed by anti-YWHAZ (green) Ab and counterstained by DAPI (blue). (H) Representative confocal images of Ki67 in tissues from ITGB1^high and ITGB1^low HCC1 cell-formed tumors. Scale bar = 100 µm. (I) The quantification of the protein expression of Ki67 by immunostaining analysis. Samples were probed by anti-Ki67 (green) Ab and counterstained by DAPI (blue). Values are presented in mean ± SD. *p < 0.05, ***p < 0.001, ****p < 0.0001.