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. 2021 Dec 17;9:802457. doi: 10.3389/fcell.2021.802457

FIGURE 1.

FIGURE 1

Single-cell RNA-seq transcriptome profiling of hRPE (A). A schematic diagram of the extracted sample cells is shown. Single cells were extracted from human RPE tissue to complete next-generation sequencing (B). Identification of cell populations. UMAP projection of 9,302 single cells from three samples showed a total of eight cell type populations. Each point is a cell, and different cell types are differentiated by different colors (C). Heatmaps of cell types. Columns represent groups of cells, and rows represent specific, highly expressed differentially expressed genes for each cell type. The heatmap scale represents the normalized expression value (D). Differential gene expression in large and small clusters is shown in the UMAP plot (E). The proportion of each sample in each cluster (F). The proportion of each cluster in each sample (G). The expression patterns of differential genes in our data were consistent with those in previous studies (H). Gene Ontology (GO) terms associated with genes with upregulated expression in the macula cluster.