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. 2021 Dec 17;9:789046. doi: 10.3389/fcell.2021.789046

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Copyright © 2021 Funakoshi, Shito, Oka and Hotta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ascidiella aspersa embryos at neurula period. Embryos are stained with Alexa Fluor™ 546 Phalloidin. Three-dimensional reconstructed images at (A) Stage 14 (early neurula), (B) Stage 15 (mid neurula), and (C) Stage 16 (late neurula). Three-dimensional reconstructed images at (A) Stage 14 (early neurula), (B) Stage 15 (mid neurula), and (C) Stage 16 (late neurula). (A–C) show the dorsal views of the embryos. (A′–C′) show the cross-section, and (A′′–C′′) show longitudinal section of (A–C), respectively. Embryo direction is shown by A, anterior; P, posterior; D, dorsal, and V, ventral. Arrowheads indicates the anterior edge of neural tube closure. en, endoderm; epi, epidermis; mech, mesenchyme; mu, muscle; noto, notochord; n.p., neuropore. Scale bar: 50 μm.