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. 2021 Dec 17;9:789046. doi: 10.3389/fcell.2021.789046

FIGURE 8.

FIGURE 8

Ascidiella aspersa embryos at the initial part of larva period. Embryos were stained with Alexa Fluor™ 546 Phalloidin. Three-dimensional reconstructed images at (A) Stage 26 (hatching larva). (A) Lateral view of the embryo. (B) Frontal view of the embryo. (C) Enlarged image of the area inside the yellow frame in (A). (D,F) Cross-section of the trunk. The positions of the sections are indicated by dashed lines in (A,B). (E) Dorsal view of the trunk. (G) Longitudinal section of the trunk. Embryo direction is shown by A, anterior; P, posterior; D, dorsal; V, ventral. Arrowheads indicate the cilia of epidermal sensory neurons. Asterisks indicates the papillae by *l: left papilla, *r: right papilla, *v: ventral papilla. asp, atrial siphon primordium; bc, brain cavity; en, endoderm; epi, epidermis; es, endodermal strand; gp, gut primordium; mech: mesenchyme; mu, muscle; nhd, neurohypophyseal duct; noto, notochord; n.t., neural tube; oc, ocellus; osp, oral siphon primordium; ot, otolith; pha, pharynx; pl, preoral lobe; and va, vacuole. Scale bar: 50 μm.