REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat anti-mouse B220 (RA3-6B2), APC	BioLegend	Cat# 103212; RRID: AB_312997
Rat anti-mouse B220 (RA3-6B2), PE/Cy5	BioLegend	Cat# 103210; RRID: AB_312995
Armenian Hamster anti-mouse CD3ε (145-2C11), APC	BioLegend	Cat# 100312; RRID: AB_ 312677
Armenian Hamster anti-mouse CD3ε (145-2C11), Biotin	BioLegend	Cat# 100304; RRID: AB_ 312669
Armenian Hamster anti-mouse CD3ε (145-2C11), PE/Cy5	BioLegend	Cat# 100310; RRID: AB_312675
Rat anti-mouse CD4 (L3T4), APC/Cy7	BioLegend	Cat# 100526; RRID: AB_312727
Rat anti-mouse CD4 (GK1.5), Biotin	BioLegend	Cat# 100404; RRID: AB_312689
Rat anti-mouse CD4 (H129.19), FITC	BioLegend	Cat# 130308; RRID: AB_1279237
Rat anti-mouse CD8a (53-6.7), APC/Cy7	BioLegend	Cat# 100714; RRID: AB_ 312747
Rat anti-mouse CD8a (53-6.7), PE	BioLegend	Cat# 100708; RRID: AB_ 312747
Rat anti-mouse CD11b (M1/70), Biotin	BioLegend	Cat# 101204; RRID: AB_312787
Rat anti-mouse CD11b (M1/70), PE	BioLegend	Cat# 101208; RRID: AB_312791
Rat anti-mouse CD11b (M1/70), PE/Cy5	BioLegend	Cat# 101210; RRID: AB_312793
Mouse anti-mouse CD45.1 (A20), Brilliant Violet 510™	BioLegend	Cat# 110741; RRID: AB_2563378
Rat anti-mouse CD45.1 (A20), PE/Cy7	BioLegend	Cat# 110730; RRID: AB_1134168
Rat anti-mouse CD45.1 (A20), PerCP	BioLegend	Cat# 110726; RRID: AB_893345
Mouse anti-mouse CD45.2 (104), APC	BioLegend	Cat# 109814; RRID: AB_389211
Mouse anti-mouse CD45.2 (104), FITC	BioLegend	Cat# 109806; RRID: AB_313443
Mouse anti-mouse CD45.2 (104), Brilliant Violet 421™	BioLegend	Cat# 109832; RRID: AB_2565511
Mouse anti-mouse CD45.2 (104), Brilliant Violet 785™	BioLegend	Cat# 109839; RRID: AB_2562604
Mouse anti-mouse CD45.2 (104), PE	BioLegend	Cat# 109808; RRID: AB_313445
Rat anti-mouse CD48 (HM48-1), APC	BioLegend	Cat# 103412; RRID: AB_571997
Rat anti-mouse CD48 (HM48-1), FITC	BioLegend	Cat# 103404; RRID: AB_313019
Rat anti-mouse CD150 (TC15-12F12.2), PE/Cy7	BioLegend	Cat# 115914; RRID: AB_439797
Rat anti-mouse CD244.2 (m2B4), PE	BioLegend	Cat# 133508; RRID: AB_2072855
Rat anti-mouse c-kit (2B8), APC	BioLegend	Cat# 105812; RRID: AB_313221
Rat anti-mouse c-kit (2B8), APC/Cy7	BioLegend	Cat# 105826; RRID: AB_1626278
Armenian Hamster anti-mouse FcεRIα (MAR-1), FITC	BioLegend	Cat# 134306; RRID: AB_1626108
Rat anti-mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5), PE	BioLegend	Cat# 108408; RRID: AB_313373
Rat anti-mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5), PE/Cy5	BioLegend	Cat# 108410; RRID: AB_313375
Rat anti-mouse Ly-6A/E (Sca-1) (D7), FITC	BioLegend	Cat# 108106; RRID: AB_313343
Rat anti-mouse Ly-6A/E (Sca-1) (D7), Brilliant Violet 421™	BioLegend	Cat# 108127; RRID: AB_2563064
Rat anti-mouse Ter119 (TER119), Biotin	BioLegend	Cat# 116204; RRID: AB_313705
Rat anti-mouse Ter119 (TER119), PE/Cy5	BioLegend	Cat# 116210; RRID: AB_313711
Streptavidin-APC	BioLegend	Cat# 405207
Streptavidin-Brilliant Violet 605™	BD	Cat# 563260
Rat anti-mouse CD34 (RAM34), FITC	eBioscience	Cat# 11-0341-85; RRID: AB_465021
Rat anti-mouse CD201 (eBio1560), APC	eBioscience	Cat# 17-2012-82; RRID: AB_10717805
Rat anti-mouse c-kit (2B8), APC-eFluor 780	Thermo Fisher Scientific	Cat# 47-1171-82; RRID: AB_1272177
Rat anti-mouse CD8a (53-6.7), Biotin	TONBO	Cat# 20-0081-U100
Chemicals, peptides, and recombinant proteins
7-Amino-Actinomycin-D (7AAD)	Sigma-aldrich	Cat# A9400-1MG
CD117 MicroBeads, mouse	Miltenyi Biotec	Cat# 130-091-224
Recombinant Murine stem cell factor (mSCF)	PEPROTECH	Cat# 250-03-10 μg
Recombinant Mouse thrombopoietin (mTPO)	PEPROTECH	Cat# AF-315-14-10 μg
Recombinant Human thrombopoietin (hTPO)	PEPROTECH	Cat# 300-18-10μg
2-Mercaptoethanol	Sigma-aldrich	Cat# M6250-100ML
Ham’s F-12 Nutrient Mix	Gibco	Cat# 11765054
StemSpan™ SFEM	Stem Cell Technologies	Cat# 09650
HEPES	Thermo Fisher Scientific	Cat# 15630080
Penicillin–Streptomycin–Glutamine	Thermo Fisher Scientific	Cat# 10378016
Insulin–Transferrin–Selenium–Ethanolamine (ITS-X)	Thermo Fisher Scientific	Cat# 51500056
Polyvinyl alcohol (87–90%-hydrolyzed)	Sigma-Aldrich	Cat# P8136-250G
Taurochenodeoxycholic acid	Sigma-Aldrich	Cat# T6260
Taurocholic acid	Sigma-Aldrich	Cat# T9034
Tauro-α-muricholic acid	Toronto Research Chemicals	Cat# T009130
Tauro-β-muricholic acid	Santa Cruz	Cat# sc-361829
Tauroursodeoxycholic acid	Sigma-Aldrich	Cat# T0266
Critical commercial assays
RNeasy Micro Kit	QIAGEN	Cat# 74004
Ki-67 staining Kit	BD	Cat# 556026
Click-IT™ L-Homopropargylglycine (HPG)	Thermo Fisher Scientific	Cat# C10186
Click-iT™ Plus Alexa Fluor™ 488 Picolyl Azide Toolkit	Thermo Fisher Scientific	Cat# C10641
BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit	Thermo Fisher Scientific	Cat# BDB554714
Deposited data
Microarray analysis	NCBI Gene Expression Omnibus (GEO) database	Accession number: GSE162408
Experimental models: Organisms/strains
Mouse: C57BL/6 (CD45.2)	Taconic	N/A
Mouse: B6.SJL (CD45.1)	In-house breeding	N/A
Oligonucleotides
Quantitative real-time PCR probe for Cpa3	Thermo Fisher Scientific	Mm00483940_m1
Quantitative real-time PCR probe for Fcer1a	Thermo Fisher Scientific	Mm00438867_m1
Quantitative real-time PCR probe for Fgd5	Thermo Fisher Scientific	Mm01189735_g1
Quantitative real-time PCR probe for Fhl1	Thermo Fisher Scientific	Mm04204611_g1
Quantitative real-time PCR probe for Gzmb	Thermo Fisher Scientific	Mm00442834_m1
Quantitative real-time PCR probe for Hlf	Thermo Fisher Scientific	Mm00723157_m1
Quantitative real-time PCR probe for Hprt	Thermo Fisher Scientific	Mm03024075_m1
Quantitative real-time PCR probe for Ldhb	Thermo Fisher Scientific	Mm01267402_m1
Quantitative real-time PCR probe for Mpl	Thermo Fisher Scientific	Mm00440310_m1
Software and algorithms
Gene Expression Commons	RIKEN	https://gexc.riken.jp/
FlowJo	Tree Star	N/A
ELDA	WEHI	https://bioinf.wehi.edu.au/software/elda/
Prism	Graphpad	N/A