Figure 6.
circMED27-knockdown-mediated biological function was restored by miR-655-3p inhibition
(A) Relative levels of miR-655-3p were detected in PLC/PRF/5 and HCCLM3 cells after being transfected with a miR-655-3p inhibitor or negative control (NC) using quantitative real-time RT-PCR. (B) Reduced circMED27 expression in PLC/PRF/5 and HCCLM3 cells increased their sensitivity to lenvatinib. (C) The expression of miR-655-3p in 150 cases of HCC tissues was detected by quantitative real-time RT-PCR. A negative correlation between circMED27 and miR-655-3p was observed in tumor tissues (R2 = 0.3842; p < 0.001). (D) The expression of USP28 in 150 cases of HCC tissues was detected by immunohistochemistry. A positive correlation between circMED27 and USP28 was observed in tumor tissues (R2 = 0.4667; p < 0.001). (E) Models of high-level circMED27 on lenvatinib resistance. The data are represented as the mean ± SD. ∗∗p < 0.01.