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. 2021 Dec 9;25(1):103593. doi: 10.1016/j.isci.2021.103593

Figure 1.

Figure 1

GS-CA1 does not require the expression of CPSF6 in CD4+ T cells to inhibit HIV-1 infection

(A) Loss of CPSF6 expression using the CRISPR/Cas9 system in human primary CD4+ T cells from two different donors was determined by western blots using antibodies against CPSF6. Controls included gRNA against CXCR4 and a non-targeting gRNA. Expression of GAPDH was the control for equal loading.

(B) Human primary CD4+ T cells depleted for CPSF6 expression were infected with HIV-1NL4-3-GFP with the indicated concentrations of PF74, GS-CA1, or DMSO as a vehicle control. Infected GFP-positive cells were determined at 72 h post-infection. Experiments were performed in triplicates, and the mean infections ±SD are shown. Results for two different donors are shown.