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. 2021 Dec 9;25(1):103593. doi: 10.1016/j.isci.2021.103593

Figure 2.

Figure 2

GS-CA1 prevents the binding of CPSF6 and Nup153 to the HIV-1 core

Human HEK293T cells were transfected with plasmids expressing CPSF6-FLAG (A) or Nup153-HA (B), and at 24 h post-transfection lysed cell extracts (INPUT) and stabilized wild-type HIV-1 capsid tubes were mixed with PF74 or GS-CA1 for 1 h before collection of the HIV-1 capsid tubes pellets by centrifugation. Pellets were washed two times and analyzed (BOUND). The input and bound fractions were analyzed by western blotting (a representative Western blot is shown) using anti-FLAG (A), anti-HA (B), or anti-p24 capsid antibodies. Experiments were repeated at least three times and a representative figure is shown. The amount of capsid relative to the total for three independent experiments is shown. Mean bound fractions relative to input ±SD are shown. ∗ indicates p value < 0.005, ∗∗ indicates p value < 0.001, ∗∗∗ indicates p value < 0.0005, as determined by using the unpaired t test.