Figure 4.

GS-CA1 does not inhibit the entry of the viral core into the nucleus

(A and B) Human A549 cells were infected with wild-type HIV-1-GFP (pseudotyped with VSV-G) at MOI = 2 with PF74, GS-CA1, or DMSO as a vehicle control. (A) Capsids from the nuclear and cytosolic fractions of cells infected at 37°C for 8 h cells were determined using western blots with anti-p24 antibodies. Nopp140 and tubulin, determined using western blots with appropriate antibodies, were markers for nuclear and cytosolic content, respectively. Nuclear and cytosolic fractions of p24 were determined by western blots from three independent experiments (a representative Western blot is shown). The mean nuclear fractions relative to the cytosolic fractions ±SD are shown. ∗∗∗∗ indicates p value < 0.0001 as determined by using the unpaired t test. (B) Infected GFP-positive cells were determined at 48 h post-infection. Infections were performed in triplicates, and the mean infections ±SD are shown.