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. 2021 Dec 9;25(1):103593. doi: 10.1016/j.isci.2021.103593

Figure 6.

Figure 6

GS-CA1 changes the conformation of the capsid

Cell extracts of Human Jurkat cells (INPUT) were mixed for 1 h at room temperature with wild-type or mutant N74D HIV-1 capsid tubes in the presence of PF74, GS-CA1, or DMSO as a vehicle control. Stabilized HIV-1 capsid tubes were collected by centrifugation and washed two times (BOUND). The input and bound fractions were analyzed by western blotting using anti-CPSF6 and anti-p24 antibodies for three experiments (a representative Western blot is shown). Mean bound fractions relative to input ±SD are shown. ∗∗∗∗ indicates p value < 0.0001, NS indicates not significant as determined by using the unpaired t test.