REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-HIV-1 p24 (183-H12-5C)	NIH AIDS Reagent Program	Cat# 3537; RRID:AB_2832923
Mouse monoclonal anti- CPSF6 (human) (3F11)	Novus	Cat# H00011052-M10; RRID:AB_2084564
Mouse monoclonal anti-SC-35 (1SC-4F11)	Sigma-Aldrich	Cat# 04-1550; RRID:AB_11212756
Mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	Invitrogen (Thermo Fisher Scientific)	Cat# AM4300; RRID:AB_437392
Rabbit polyclonal anti-alpha Tubulin	Invitrogen	Cat# PA5-29444; RRID:AB_2546920
Mouse monoclonal anti-FLAG M2	Sigma-Aldrich	Cat# F1804; RRID:AB_262044
Mouse monoclonal anti-hemagglutinin (HA)	Sigma-Aldrich	Cat# H3663; RRID:AB_262051
Rabbit polyclonal anti-Nopp140 (human) (RS8 serum)	(Kittur et al., 2007)	Gift of U. Thomas Meier, Albert Einstein College of Medicine
Goat anti-Mouse IRDye 680LT	LI-COR	Cat# 925-68020; RRID:AB_268782
Goat anti-Rabbit IRDye 680LT	LI-COR	Cat# 925-68021; RRID:AB_2713919
Goat anti-Mouse IRDye 800CW	LI-COR	Cat# 926-32210; RRID:AB_621842
Goat anti-Rabbit IRDye 800CW	LI-COR	Cat# 925-32211; RRID:AB_621843
Bacterial and virus strains
E. Coli DH5α competent cells	Zymo Research	Cat# T3007
E. Coli One Shot BL21star (DE3) cells	Thermo Fisher Scientific	Cat# C601003
HIV-1 NL4-3	(Selyutina et al., 2020b)	NA
HIV-1-P38S	This paper	NA
HIV-1-S41A	This paper	NA
HIV-1-P38S/S41A	This paper	NA
Chemicals, peptides, and recombinant proteins
Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	Cat#: D2438; CAS: 67-68-5
Difco LB Broth, Miller (Luria-Bertani)	Fisher Scientific	Cat#: BD244610
Tris (TRIS(HYDROXYMETHYL)AMINOMETHANE)	Crystalgen	Cat#: 300-844-5000; CAS: 77-86-1
cOmplete EDTA-free protease inhibitor cocktail	Millipore Sigma	Cat#: 11873580001
Sodium Chloride (NaCl)	Crystalgen	Cat# 300-747-5000; CAS: 767-14-5
β-Mercaptoethanol (BME)	Acros organics	Cat# 125470010; CAS: 60-24-2
2-(N-morpholino) ethanesulfonic acid (MES)	Calbiochem	Cat# 475893; CAS: 4432-31-9
Magnesium Chloride (MgCl2)	Sigma-Aldrich	Cat# M2670; CAS: 7791-18-6
Potassium Chloride (KCl)	Fisher Scientific	Cat# BP366-1; CAS: 7447-40-7
Dithiothreitol (DTT)	VWR	Cat# 97061-340; CAS: 3483-12-3
D-(+)-Sucrose	VWR	Cat# 97061-432; CAS: 57-50-1
Dulbecco's Phosphate-Buffered Salt (PBS) Solution 1X	Corning	21031CV
EDTA, pH 8.0, 0.5M	Corning	46034CI
Paraformaldehyde (4% in PBS)	Boston BioProducts	Cat# BM-155
PF74	Sigma Aldrich	Cat# SML0835
Nevirapine	NIH AIDS Reagent Program	Cat# 4666
Triton X-100	Sigma	Cat# T-9284 CAS: 9002-93-1
Nonidet P40 Substitute (NP40)	Fluka Biochemika	Cat# 74385 CAS: 9016-45-9
Glycerol	Millipore	Cat# 356350-1000ML CAS: 56-81-5
Isopropyl β-D-1-thiogalactopyranoside (IPTG)	Teknova	Cat# I3325
Bovine Serum Albumin (BSA) (Fraction V)	Fisher bioreagents	Cat# BP1600-100 CAS: 9048-46-8
Human IL-2 IS	Miltenyi Biotec	Cat# 130-097-743
Pronase	Roche Diagnostics GmbH	Cat# 41844620 CAS: 9036-06-0
Poly-L-Lysine (0.01% solution)	Sigma	Cat# P4707
GS-CA1		Generously provided by Stephen Yant (Gilead Sciences, CA)
Lenacapavir		Generously provided by Stefan G. Sarafianos
Critical commercial assays
QuikChange II site-directed mutagenesis kit	Agilent	Cat#: 200523
QIAamp DNA Micro Kit	Qiagen	Cat# 56304
Experimental models: Cell lines
Human: Jurkat	ATCC	TIB-152
Human: 293T/17	ATCC	CRL-11268
Human: A549	ATCC	CCL-185
Human: HeLa	ATCC	CCL-2
Oligonucleotides
All standard cloning primers for site-directed mutagenesis	Integrated DNA Technologies	NA
Late Reverse Transcripts (LTR) MH531: 5’-TGTGTGCCCGTCTGTTGTGT-3’	(Mbisa et al., 2009)	NA
Late Reverse Transcripts (LTR) MH532: 5’-GAGTCCTGCGTCGAGAGATC-3’	(Mbisa et al., 2009)	NA
Late Reverse Transcripts (LTR) Probe LRT-P: 5′ -(FAM)-CAGTGGCGCCCGA ACAGGGA-(TAMRA)-3’	(Mbisa et al., 2009)	NA
2-LTR circles MH5355′-AACTAGGGAACC CACTGCTTAAG-3′	(Mbisa et al., 2009)	NA
2-LTR circles MH5365′-TCCACAGATCAA GGATATCTTGTC-3′	(Mbisa et al., 2009)	NA
2-LTR probe MH6035’-(FAM)-ACACTACTTG AAGCACTCAAG-GCAAGCTTT-(TAMRA)-3′	(Mbisa et al., 2009)	NA
CPSF6 crRNA, guide # 5 GGACCACATAGACATTTACG	Dharmacon	CM-012334-05
CPSF6 crRNA, guide # 6 ATATATTGGAAATCTAACAT	Custom sequence	NA
TRIM5alpha crRNA, guide # 6 AAGAAGTCCATGCTAGACAA	Custom sequence	NA
TRIM5alpha crRNA, guide # 7 GTTGATCATTGTGCACGCCA	Custom sequence	NA
Recombinant DNA
pLPCX	Clontech	Cat# 631511
pLPCX-CPSF6(1-358)-FLAG	Fricke et al., 2013b	NA
pLPCX-Nup153-HA	Buffone et al. (2018)	NA
Plasmid: Tat	Stremlau et al., (2006)	NA
Plasmid: Rev	Stremlau et al., (2006)	NA
Plasmid: VSVg	Stremlau et al., (2006)	NA
pVPack-GP	Agilent	Cat #: 217566
pVPack-VSV-G	Agilent	Cat #: 217567
pET-11a-p24-A14C/E45C	Selyutina et al., (2018)	NA
pET-11a-p24-A14C/E45C-N74D	Selyutina et al., (2018)	NA
Other
TURBO™ DNase (2 U/μL)	Invitrogen	Cat#AM2238
Fetal Bovine Serum (FBS, heat-inactivated)	Gibco	16140-071
RPMI-1640 (high glucose)	Corning	MT10-017-CV
Penicillin–streptomycin (5 mg/mL)	Corning	MT10-040-CV
Sodium pyruvate (100x)	Corning	MT25-000-CI
HEPES (100x)	Fisher/HyClone	SH3023701
Ficoll-Paque Plus	Cytiva	17-1440-02
In Vivo Ready™ Anti-Human CD3 (UCHT1)	Tonbo biosciences	40-0038-U500
Purified Anti-Human CD28 (CD28.2)	Tonbo biosciences	70-0038-U100
DMEM (high glucose)	Corning	MT10-017-CV
HiTrap Q HP (5 mL)	Cytiva	17115401
HiTrap SP FF (5 mL)	Cytiva	17515701
Centrifugal filter units Amicon Ultra-4	Millipore	UFC801024
SnakeSkin Dialysis tubing 10K MWCO	Thermo Scientific	68100
P3 Primary Cell 96-well Nucleofector kit	Lonza	V4SP-3096
T cell Activation/Expansion Kit, human	Miltenyi Biotec	130-091-441
TaqMan Universal Probe Master Mix II, with UNG	Thermo Fischer Scientific	4440038
EasySep Human CD4+ T Cell Isolation Kit	Stemcell Technologies	Cat# 17952