Figure 2.

AS-NMD assay showed reporters' responses to each FDA drug treatment

(A–C) Expression level changes in non-NMD isoform and NMD isoform of Ptbp2 (A), Hnrnpl (B), and Tra2b (C) were shown as scatterplots. Upregulation and downregulation of reporter gene expression were indicated by the 2-fold-change lines. Each dot represented the average of three replicates of one drug treatment, and the red dot represented the averaged expression level change in TG. (D–F) Identification of drugs that altered NMD isoforms >2-fold or <0.5-fold and altered the non-NMD isoforms between 0.5- and 2-fold of Ptbp2 (D), Hnrnpl (E), and Tra2b (F). (G and H) Venn diagrams were used to identify drugs exhibiting similar responses in all three reporter genes. (G) One drug changed NMD isoforms <0.5-fold and non-NMD isoforms between 0.5- and 2-fold. (H) Two drugs changed NMD isoforms >2-fold and non-NMD isoforms between 0.5- and 2-fold. Positive control, TG, is not included in (D–H).