Figure 3.

Normalizing transcriptional responses using the NMD/non-NMD ratio of reporter genes to assess drug's impact on cellular NMD regulation

(A) Many FDA-approved drugs induced transcriptional responses of the reporter genes. (B) PCA analysis of NMD/non-NMD ratios (RQ ratios) of three reporters. (C–E) Ratio of NMD/non-NMD isoforms were calculated for Ptbp2 (C), Hnrnpl (D), and Tra2b (E) reporter genes. Red lines indicated 2-fold upregulation or downregulation of the ratios, and the red dot represented the averaged NMD/non-NMD ratio of TG.