HHT inhibits NMD in a dosage-dependent manner
(A–C) NMD isoform expression level changes in Ptbp2 (A), Hnrnpl (B), and Tra2b (C) showed a dosage-dependent manner compared with non-NMD isoform in N2a cells. HHT started to inhibit NMD as low as 0.05 μM.
(D–F) NMD isoform expression level changes in PTBP2 (D), HNRNPL (E), and TRA2B (F) showed the same dosage-dependent manner in HEK 293T cells. Data were shown as mean ± SEM of three biological replicates. ∗, p < 0.05, ∗∗, p < 0.01, Student's t test.