Figure 4.

The metabolic uptake and secretion profiles are unique in EC

Metabolite secretion and uptake analysis in EC (n = 13) and HC (n = 14).

(A–C) Receptor expression on CD4⁺ and CD8⁺ T cells of Glut-1 (A), MCT-1 (B), and xCT (C). Contour plots are representative images showing median expression profile in % of cells and graphs show the median fluorescence intensity (MFI).

(D–F) Intracellular levels of glucose (D), lactate (E), and glutamine/glutamate (Gln/Glu) ratio (F). Graphs show the relative light unit (RLU) and were performed in duplicates.

(G) Correlation analysis of intracellular glucose levels with duration of EC status.

(H and I) Correlation matrix of intracellular metabolite levels and transporter expression in EC (H) and HC (I). Statistical analysis was performed using Mann-Whitney U-test (significance level, p < 0.05) and represented with median and 95% CI or Spearman correlation (significance level, p < 0.05). See also Figure S4.