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. 2021 Dec 13;25(1):103627. doi: 10.1016/j.isci.2021.103627

Figure 4.

Figure 4

Androgens induced expression of Prkar2b and Marcks in vitro in granulosa cells isolated from RD mice

(A) Schematic representation of the androgen stimulation experiments on GCs isolated from 12- to 14-day-old RD mice. DHT, dihydrotestosterone; T, testosterone.

(B) qPCR assay of GCs under different treatments for Inhbb, Marcks, and Prkar2b. Expression levels were normalized to Rps24. Two technical duplicates were performed in individual qPCR experiments. Error bars indicate the mean ± SEM. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, n.s. denotes not significant (Wilcoxon rank-sum test).

(C and D) Immunofluorescence staining for Prkar2b (C) and Marcks (D) in cultured GCs with T or DHT treatment. Tissues were counterstained with DAPI. Scale bars are as indicated. Density plot shows immunofluorescence signal intensity in cells for Prkar2b (C) and Marcks (D) under different treatments. The signal intensity was measured with Zeiss ZEN. The mean signal intensity of specific antibody staining is indicated as a dashed line. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001 and n.s. denotes not significant. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, n.s. denotes not significant (Wilcoxon rank-sum test).