Androgen receptor antagonist treatment alleviated the imbalances of the sub-type composition in GCs from obese mice
(A) Schematic representation of the androgen receptor antagonist treatment in vivo experiments on HFD mice.
(B) Pseudotime trajectory of granulosa cells from HFD and ENZA-HFD mice. Trajectory was inferred by Monocle 2. Cells are color coded by State inferred in the pseudotime analysis.
(C) Bar plot showing percentage of cells in State 3 in HFD and ENZA-HFD mice, respectively. The Chi-squared test was performed.
(D) Immunofluorescence staining for Prkar2b and Marcks in ovarian tissues from RD (Vehicle only), HFD (Vehicle only) and ENZA-HFD mice. Tissues were counterstained with DAPI, and Ddx4 or Gdf9.