REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-LECT1 (Cnmd)	Thermo Fisher Scientific	Cat# PA5-76974; RRID: AB_2720701
Rabbit anti-Cyp17a1	Abcam	Cat# ab134910
Rabbit anti-DHT	Mybiosource	Cat# MBS2042442
Rabbit anti-Ddx4/Mvh	Abcam	Cat# ab13840; RRID: AB_443012
Rabbit anti-Gdf9	Abcam	Cat# ab254323
Mouse anti-Stc1	Santa Cruz Biotechnology	Cat# sc-293435
Mouse anti-Lhcgr	Novus	Cat# NBP2-52504; RRID: AB_2783817
Mouse anti-Marcks	Santa Cruz Biotechnology	Cat# sc-100777; RRID: AB_1125958
Mouse anti-Prkar2b	Santa Cruz Biotechnology	Cat# sc-376778
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11029; RRID: AB_2534088
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555	Thermo Fisher Scientific	Cat# A-21429; RRID: AB_2535850
Biological samples
Mouse ovarian tissue	This study	N/A
Chemicals, peptides, and recombinant proteins
M2 medium	Sigma-Aldrich	Cat# M7167
Penicillin-Streptomycin	GIBCO	Cat# 15140122
Collagenase, Type IV	GIBCO	Cat# 17104019
TrypLE Express Enzyme (1×)	GIBCO	Cat# 12604039
PMSG, Pregnant Mare Serum Gonadotropin	Solarbio	Cat# P9970
hCG, Human Chorionic Gonadotrophin	SANMA	N/A
α-MEM	GIBCO	Cat# 12561056
FBS, Fetal Bovine Serum	VisTech	Cat# SE200-ES
Insulin-Transferrin-Selenium (ITS -G) (100×)	GIBCO	Cat# 41400045
Nucleosides (100×)	Sigma-Aldrich	Cat# ES-008-D
Paraformaldehyde	Sigma-Aldrich	Cat# P6148
Sucrose	Sigma-Aldrich	Cat# V900116
Recombinant RNase Inhibitor	TaKaRa	Cat# 2313B
KAPA HiFi HotStart ReadyMix	Roche	Cat# 7958935001
Ampure XP beads	Beckman Coulter	Cat# A63882
dNTP mix	Thermo Fisher Scientific	Cat# R0192
Dynabeads M-280 Streptavidin	Thermo Fisher Scientific	Cat# 11206D
Tissue freezing medium (OCT medium)	Leica	Cat# 14020108926
Goat serum	GIBCO	Cat# 16210064
PrimeScript RT Master Mix (Perfect Real Time)	TaKaRa	Cat# RR036A
2× SYBR Green PCR Master Mix	Bimake	Cat# B21202
Dihydrotestosterone, DHT	Solarbio	Cat# ID0310
Enzalutamide (MDV3100)	Selleckchem	Cat# S1250
Testosterone	Solarbio	Cat# IT0110
PEG-400	KESHI	Cat# 25322-68-22
Tween-80	Solarbio	Cat# T8360
Triton X-100	Sigma-Aldrich	Cat# T9284
ProLong Gold Antifade Mountant with DAPI	Thermo Fisher Scientific	Cat# P36935
Critical commercial assays
Quick-RNA Microprep Kit	Zymo Research	Cat# R1051
DNA Clean & Concentrator-5 (Capped)	Zymo Research	Cat# D4014
NEBNext Ultra II DNA Library Prep Kit for Illumina	New England Biolabs	Cat# E764
Deposited data
Raw data files and processed data files for ovarian single-cell RNA-seq	This study	GEO: GSE142587
Experimental models: Organisms/strains
Mouse: C57BL/6J	Vital River	N/A
Mouse: BKS-Leprem2Cd479/Gpt	GemPharmatech	Cat# T002407
Mouse: B6/JGpt-Lepem1Cd25/Gpt	GemPharmatech	Cat# T001461
Oligonucleotides
qPCR primers, see also Table S7	This paper	N/A
Software and algorithms
ImageJ (version: 1.5.3.c)	NIH	https://imagej.nih.gov/ij/
ZEN	Zeiss	http://www.zeiss.com/microscopy/us/products/microscope-software/zen.html/
Cutadapt (version: 2.3)	Read the Docs	https://cutadapt.readthedocs.io/en/stable/
STAR (version: 2.6.0a)	Github	https://github.com/alexdobin/STAR
HTSeq (version: 0.11.2)	Read the Docs	https://htseq.readthedocs.io/en/master/history.html#version-0-11-2
Scater (version: 1.14.6)	Bioconductor	http://bioconductor.org/packages/release/bioc/html/scater.html
Seurat (version: 3.2.0)	Satija Lab	https://satijalab.org/seurat/
SCTransform (version: 0.2.1)	Satija Lab	https://satijalab.org/seurat/
clusterProfiler (version: 3.14.3)	Bioconductor	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
GSVA (version: 1.34.0)	Bioconductor	https://bioconductor.org/packages/release/bioc/html/GSVA.html
Monocle 2 (version: 2.14.0)	Bioconductor	http://cole-trapnell-lab.github.io/monocle-release
CellPhoneDB (version: 2.14.0)	Github	https://github.com/Teichlab/cellphonedb
pySCENIC (version: 0.10.3)	Van de Sande et al. (2020)	https://pyscenic.readthedocs.io/en/latest
Cytoscape (version: 3.8.0)	https://cytoscape.org	https://cytoscape.org