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. 2021 Dec 9;25(1):103592. doi: 10.1016/j.isci.2021.103592

Figure 5.

Figure 5

Onset of oral cGVHD is associated with loss of acinar-specific ZG16B and lymphocyte infiltration in minor salivary glands

(A–C) IHC staining of ZG16B (red) in labial MSG of (A) healthy volunteers (n = 5), (B) post-HSCT patients without oral cGVHD (n = 5), and (C) post-HSCT patients with oral cGVHD (n = 5). DAPI (blue) indicates nucleated cells and aquaporin 5 (green) labels the apical membrane of acinar cells. Magnification 400x, scale bars = 50 μm.

(D) Quantification of ZG16B staining was performed as described in STAR Methods. The mean and individual values are shown for n = 5 per group. A one-way ANOVA with Tukey's correction for multiple comparisons indicates significant (∗p ≤0.01) differences between the groups and specific differences between no oral cGVHD and oral cGVHD (p<05) and healthy and oral cGVHD. (E–G) IHC staining of CD45 to mark lymphocytes (red) and nuclei (blue) in labial MSG of (E) healthy volunteers (n = 5), (F) post-HSCT patients without oral cGVHD (n = 5), and (G) post-HSCT patients with oral cGVHD (n = 5). Magnification 400x, scale bars = 100 μm.

(H) Quantification of CD45+ cells was performed as described in STAR Methods. The mean and individual values are shown for n = 4 to 5 per group. A one-way ANOVA with Tukey's correction for multiple comparisons indicates significant (∗p ≤0.01) differences between the groups and specific differences between no oral cGVHD and oral cGVHD (p<01) and healthy and oral cGVHD (p<0.05). (I–K) Representative images of Masson's trichrome-stained histological sections of (I) healthy volunteers (n = 5), (J) post-HSCT patients without oral cGVHD (n = 10), and (K) post-HSCT patients with oral cGVHD (n = 10) labial MSG from cohort 2 are shown. Blue staining marks collagen deposition.

(L) Quantification of MSG fibrosis area adjusted by total area from Masson trichrome-stained sections shown as individual values with mean. A one-way ANOVA with Tukey's correction for multiple comparisons indicate no significant (∗p ≤0.05) differences between the groups Magnification 20x, scale bars = 200 μm. See also Figure S4.