| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat Polyclonal anti-AQP5 (G19) | Santa Cruz | Cat# sc-9890, RRID:AB_2059877 |
| Rabbit Monoclonal Anti-Calmodulin (EP799Y) | Abcam | Cat# ab45689, RRID:AB_725815 |
| Rabbit Polyclonal anti-Ezrin | Cell Signaling | Cat# 3145S, RRID:AB_2100309 |
| Rat Monoclonal anti-KRT19 (TROMA-3) | Sigma-Aldrich | Cat# MABT913, RRID:AB_2892523 |
| Rabbit Monoclonal anti-MMP9 (D6O3H) | Cell Signaling | Cat# 13667, RRID:AB_2798289 |
| Rabbit Monoclonal Anti-GCDFP 15 (PIP, EP1582Y) | Abcam | Cat# ab62363, RRID:AB_940649 |
| Rabbit Polyclonal anti-MUC7 | Atlas Antibodies | Cat# HPA006411, RRID:AB_1854204 |
| Mouse Monoclonal anti-A1ACT (a1ACT; 71A1) | Thermo Fisher Scientific | Cat# LF-MA0166, RRID:AB_1954828 |
| Rabbit Polyclonal anti-CD45 | Abcam | Cat# ab10558, RRID:AB_442810 |
| Rabbit Polyclonal anti-ZG16B | Sigma-Aldrich | Cat# HPA041125-100UL; RRID:AB_10794264 |
| Alexa Fluor 488-AffiniPure Bovine Anti-Goat IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 805-545-180, RRID:AB_2340883 |
| Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 711-585-152, RRID:AB_2340621 |
| Alexa Fluor 647-AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 712-605-153, RRID:AB_2340694 |
| IRDye 800CW Donkey anti-Rabbit IgG antibody | Li-COR Biosciences | Cat# 926-32213, RRID:AB_621848 |
| IRDye 680RD Donkey anti-Mouse IgG antibody | Li-COR Biosciences | Cat# 926-68072, RRID:AB_10953628 |
| Biological samples | ||
| Patient samples (saliva and MSG biopsies) | NIH Clinical Center | NIH IRB-approved, Clinicaltrials.gov registered protocols: NCT01851382, NCT00092235, and NCT03602599 |
| Chemicals, peptides, and recombinant proteins | ||
| Trypsin, TPCK treated, 10-Pack | AB Sciex | Cat# 4352157 |
| Xylene Substitute | Sigma-Aldrich | Cat# A5597-1GAL |
| Normal donkey serum | Jackson ImmunoResearch Labs | Cat# 017-000-121 |
| Antibody diluent | DAKO | Cat# S0809 |
| Fluoromount-G | Thermo Fisher Scientific | Cat# 00-4958-02 |
| DAPI | Thermo Fisher Scientific | Cat# 62248 |
| Collagenase, type 2 | Worthington | Cat# LS004177 |
| Deoxyribonuclease I from bovine pancreas | Sigma-Aldrich | Cat# DN25-1G |
| PBS 1X | Quality Biological, Inc | |
| Odyssey Blocking Buffer (TBS) | Li-COR Biosciences | Cat# 927-50000 |
| 10X TBS pH 7.4 | Quality Biological | Cat# 351-086-101 |
| 4X Protein Sample Loading Buffer | Li-COR Biosciences | Cat# 928-40004 |
| Heat Inactivated Fetal Bovine Serum (FBS) | Quality Biological, Inc | Cat# 110-001-101HI |
| RPMI 1640 | GIBCO | Cat# 61870127 |
| Penicillin-Streptomycin | GIBCO | Cat# 15140122 |
| Tween 20 | Quality Biological, Inc | Cat# A611-M147-13 |
| Critical commercial assays | ||
| Pierce BCA protein assay kit | Thermo Fisher Scientific | Cat# 23227 |
| iTRAQ Reagent-8Plex Multiplex Kit | AB Sciex | Cat# 4390812 |
| iTRAQ Reagent- Multiplex Buffer Kit | AB Sciex | Cat# 4381664 |
| PNGase F | New England Biolabs | Cat# P0704L |
| Masson’s Trichrome 2000Stain Kit | StatLab | Cat# KTMTR2 EA |
| Zenon Alexa Fluor 594 Rabbit IgG labeling kit | Thermo Fisher Scientific | Cat# Z25307 |
| Deposited data | ||
| scRNAseq of healhy volunteer MSG | This paper | GEO record GSE180544 |
| Saliva Proteomics | This paper | Center for Open Science OSF project “Salivary proteomics in chronic graft-versus-host disease” |
| Salivary ZG16B decreased in oral cGVHD_raw LiCor Western blot scans | This paper | Center for Open Science OSF project “Salivary proteomics in chronic graft-versus-host disease” are available from Mendeley Data at doi:10.17632/7g5cftcbpv.1 |
| Software and algorithms | ||
| ImageJ 1.52a | (Schindelin et al., 2012; Schneider et al., 2012) | https://imagej.nih.gov/ij; RRID:SCR_003070 |
| Biorender | Biorender | https://biorender.com/; RRID:SCR_018361 |
| GraphPad Prism 8.4.1 | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
| Proteome Discoverer (v. 1.4) | Thermo Fisher Scientific | Proteome Discoverer, RRID:SCR_014477 |
| Scaffold Q+ (v. 4.3.0) | Proteome Software | N/A |
| Protein Prophet | (Nesvizhskii et al., 2003) | N/A |
| i-Tracker (v.1.1) | (Shadforth et al., 2005) | N/A |
| Ingenuity Pathway Analysis | QIAGEN | RRID:SCR_008653 |
| Image Studio Lite (v.5.2.5) | Li-COR Biosciences | https://www.licor.com/bio/image-studio-lite/; RRID:SCR_013715 |
| Volocity 6.3 | PerkinElmer | https://www.perkinelmer.com/lab-solutions/resources/docs/BRO_VolocityBrochure_PerkinElmer.pdf; RRID:SCR_002668 |
| Adobe Photoshop CC | Adobe Systems | https://www.adobe.com/products/photoshop.html; RRID:SCR_014199 |
| Cell Ranger Software Suite (v.3.0.1) | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation; RRID:SCR_016957 |
| Seurat R Package (v.3) | (Stuart et al., 2019) | https://satijalab.org/seurat/; RRID:SCR_016341 |
| Clustree R package | (Zappia and Oshlack, 2018) | https://cran.r-project.org/web/packages/clustree/index.html ; RRID:SCR_016293 |
| Enrichr | (Chen et al., 2013) | https://maayanlab.cloud/Enrichr/; RRID: SCR_001575 |
| STRING (v.10) | (Szklarczyk et al., 2017) | https://string-db.org/; RRID:SCR_005223 |
| SoupX | (Young and Behjati, 2020) | https://github.com/constantAmateur/SoupX; RRID:SCR_019193 |
| Other | ||
| SwissProt | UniProtKB/Swiss-Prot | https://www.expasy.org/proteomics/; RID:SCR_004426 |
| Mini Gel Tank and Blot Module Set | Thermo Fisher Scientific | Cat# NW2000 |
| Revert™ 700 Total Protein Stain | Li-COR Biosciences | Cat# 926-11011 |
| NuPAGE™ 4 to 12%, Bis-Tris | Thermo Fisher Scientific | Cat# NP0323BOX |
| MACS SmartStrainers (70 μm) | Miltenyi Biotec | Cat# 130-110-916 |
| GentleMACS C tubes | Miltenyi Biotec | Cat#130-093-237 |
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