Analysis of mRNA expression. (a) Detection of IL-12p40, IL-12p35, and IL-23p19 mRNA expression in peripheral blood of ITP patients (n = 30) and healthy subjects (n = 20) by RT-PCR. (b) Detection of T-bet, GATA-3, RORγt, and Foxp-3 expression in peripheral blood of ITP patients (n = 30) and healthy subjects (n = 20) by RT-PCR.
IL: interleukin; ITP: primary immune thrombocytopenia.