Figure 3.

Dynamics of the consumption of O₂ dissolved in the growth medium by E. coli ML-35p. Molecular oxygen consumption was measured in the presence of AgNP-AMP/AP conjugates or antimicrobial peptides and proteins (AMPs and APs) and gelatin-only coated silver nanoparticles (AgNPs) alone. The concentration of dissolved oxygen was measured using an Oxigraph Plus Clark-type electrode unit with hermetically sealed lid (Hansatech Instruments ltd, UK). The concentration of antimicrobials in the test mixture was equal to 4 × MIC (minimal inhibitory concentration); typical curves are shown.