Table 2.
Antimicrobial activity of silver nanoparticle conjugates with antimicrobial peptides or proteins.
| Bacteria | MICa (μg/ml) | MBCa (μg/ml) | |||||
|---|---|---|---|---|---|---|---|
| AgNPb | AgNP-PG1 | AgNP-Ind | AgNP-Prot | AgNP-His | AgNP-Lyz | AgNP-Lyz | |
| Gram-positive | |||||||
| S. aureus SG511 | 12 | 6 | 6 | 6 | 12 | 6 | 6 |
| S. aureus ATCC 25923 | 12 | 6 | 12 | 6 | 24 | 12 | 12 |
| S. aureus 1399/17 clin.is. | 48 | 6* | 12* | 24 | 48 | 24 | 24 |
| S. intermed. clin.is | 48 | 6* | 6* | 6* | 12* | 24 | 24 |
| MRSA ATCC 33591 | 48 | 6* | 12* | 24 | 48 | 24 | 24 |
| Gram-negative | |||||||
| P. aerugin. АТСС 27853 | 24 | 6* | 12 | 6* | 12 | 12 | 12 |
| P. aerugin. clin.is. | 24–48 | 12~* | 12~* | 12~* | 24 | 12–24 | 24 |
| Klebsiella spp. clin.is. | 24 | 6* | 12 | 12 | 12 | 6* | 12 |
| E. coli ATCC 25922 | 24 | 6 | 12 | 12 | 24 | 6* | 12 |
| E. coli ML-35p | 24 | 6 | 12 | 12 | 24 | 6* | 6 |
| GMIC | 26.8 | 6.4 | 10.4 | 10.4 | 20.9 | 11.7 | — |
| PG-1 | Indolicidin | Protamine | Histones | Lysozyme | |||
| Gram-negative | |||||||
| E. coli ML-35p | — | 6.7 | 10.3 | 10.5 | 76 | >3,500 | |
| Klebsiella spp. clin.is. | — | 10 | >40 | 40 | >150 | >400 | |
| P. aerugin. clin.is. | — | 5 | 10 | 5 | 38 | >400 | |
| P. aerugin. АТСС 27853 | — | 5 | 10 | 5 | 38 | >400 | |
| Gram-positive | |||||||
| MRSA ATCC 33591 | — | 8.5 | 40 | 20 | >300 | >3,500 | |
| S. intermed. clin.is | — | 10 | >40 | 20 | >300 | >400 | |
| S. aureus 1399/17 clin.is | — | 5 | 40 | 20 | >300 | >400 | |
| S. aureus ATCC 25923 | — | 10 | 40 | 40 | >300 | >400 | |
| GMIC | — | 7.2 | >23.9 | 15.5 | >138.2 | >688.0 | |
a
Minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) are the medians of 3–4 independent experiments carried out in triplicate. GMIC is the geometric mean of all the evaluated MICs.
b
Nanoparticles coated only with gelatin.
*
> 4-fold reduction of MIC compared with that of gelatin-only coated AgNPs.