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. 2020 Dec 10;107(1):154–166. doi: 10.3324/haematol.2019.239186

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Figure 7. — Deletion of Nupr1 in adulthood promotes hematopoietic stem cell engraftment. (A) Cell cycle analysis of Nupr1^fl/flMx1-cre hematopoietic stem cells (HSC) under homeostasis. Representative plots of cell cycle from representative wild-type (WT) and Nupr1^fl/flMx1-cre mice (8 weeks old). WT littermates (8 weeks old) were used as controls. HSC (Lin^– (i.e., CD2, CD3^– CD4^– CD8^– B220^– Gr1^– CD11b^– Ter119^–) CD48^– Sca1⁺ c-kit⁺ CD150⁺ CD34^– CD135^–) were analyzed by DNA content (DAPI) versus Ki-67. G0 (Ki-67^lowDAPI^2N), G1 (Ki-67^highDAPI^2N), G2-S-M (Ki-67^highDAPi>^2N-4N). (B) Statistical analysis of the number of long-term HSC from WT and Nupr1^fl/flMx1- cre mice. BMNC: bone marrow nucleated cells. (C) Statistical analysis of the cell cycle of HSC. Ctr: n=4, Nupr1^fl/flMx1-cre, n=5. **P<0.01. (D) Kinetic analysis of donor chimerism (CD45.2⁺) in peripheral blood. Data were analyzed by two-way analysis of variance and are represented as mean ± SD (Ctr group: n = 5 mice, Nupr1^fl/flMx1-cre group: n =7 mice). ***P<0.001. (E) Flow cytometry analysis of the HSC compartment in primary recipients 4 months after transplantation. (F) Statistical analysis of donor HSC number and percentage in the transplantation chimeras. Data were analyzed using an unpaired Student t-test and are represented as mean ± standard deviation, n=5. **P<0.01, ***P<0.001.