FIG. 1.

Amplification of DNA fragments from different Brucella strains. DNA was amplified by the abbreviated AMOS PCR assay described in the text. Eight microliters of amplicons was separated by electrophoresis, treated with ethidium bromide, and visualized under UV light. Strain 544 is biovar 1; B. abortus S19 in lane 4 is from a lab passage; B. abortus S19 in lanes 5 to 7 was reisolated from vaccinated cattle. For other species, the strains used were as follows: B. canis strain RM 6/66, B. melitensis bv. 1 strain 16M, B. neotomae strain 5K33, B. ovis strain 63/290, and B. suis bv. 1 strain 1330.