REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SARS-CoV-2 S2, human IgG1	Sino Biological Inc	CAT#40590-D001; RRID:AB_2857932
MERS-CoV S R723, rabbit	Sino Biological Inc	CAT#40069-R723; RRID:AB_2860455
MERS-CoV S T62, rabbit	Sino Biological Inc	CAT#40069-T62; RRID:AB_2904183
MERS-CoV S MM23, mouse	Sino Biological Inc	CAT#40069-MM23; RRID:AB_2860454
ACE2, rabbit	Abcam	CAT#ab15348; RRID:AB_301861
β-actin, mouse	Sigma	CAT#A5316; RRID:AB_476743
p24, rabbit	Sino Biological Inc	CAT#11695-RP02; RRID:AB_2904185
anti-CD16 conjugated with BV421, mouse IgG1	BD Biosciences	CAT#562878; RRID:AB_2737861
anti-CD32 conjugated with FITC, mouse IgG1	BD Biosciences	CAT#552883; RRID:AB_394512
anti-CD64 conjugated with APC, mouse IgG1	BD Biosciences	CAT#561189; RRID:AB_10611566
control mouse IgG1 conjugated with BV421	BD Biosciences	CAT#562438; RRID:AB_11207319
control mouse IgG1 conjugated with FITC	BD Biosciences	CAT#555748; RRID:AB_396090
control mouse IgG1 conjugated with APC	BD Biosciences	CAT#554681; RRID:AB_398576
CD16, mouse	BD Biosciences	CAT#555404; RRID:AB_395804
CD32, mouse	BD Biosciences	CAT#555447; RRID:AB_395840
CD64, mouse	BD Biosciences	CAT#555525; RRID:AB_395911
anti-CD16 conjugated with Pacific blue	Beijing 4A Biotech	CAT#FHW016-100
HRP-conjugated goat anti-human IgG	Solarbio	CAT#SE101; RRID:AB_2904186
goat anti-human IgG conjugated with PE	Abcam	CAT#ab98596; RRID:AB_10673825
IRDye® 800CW Goat anti-Mouse IgG	LI-COR Biosciences	CAT#925-32210; RRID:AB_2687825
IRDye® 680RD Goat anti-Rabbit IgG	LI-COR Biosciences	CAT#925-68071; RRID:AB_2721181
anti-SARS-CoV-2 CB6, human IgG1	this paper	N/A
anti-SARS-CoV-2 CB6, human IgM	this paper	N/A
anti-SARS-CoV-2 CB6, human IgA	this paper	N/A
anti-SARS-CoV-2 N1G12, human IgG1	this paper	N/A
mouse serum anti-SARS-CoV-2 S1	this paper	N/A
anti-SARS-CoV-2 XG005	Dr. Qiao Wang	N/A
anti-EBOV ADI15878	Dr. Linqi Zhang	N/A
anti-EBOV 206	Dr. Linqi Zhang	N/A
anti-EBOV 314	Dr. Linqi Zhang	N/A
anti-MARV MR191	Dr. Linqi Zhang	N/A
Chemicals, peptides, and recombinant proteins
EK1C4	Dr. Shibo Jiang & Dr. Lu Lu	N/A
control peptide: YDHTKNYPFDVDQ	this paper	N/A
sACE2	this paper	N/A
Critical commercial assays
Vironostika HIV-1 Antigen MicroELISAKit	Biomerieux bv, Boxtel	CAT#VPK-107
Experimental models: Cell lines
293T	ATCC	CAT#CRL-3216
THP-1	ATCC	CAT#TIB-202
Raji	ATCC	CAT#CCL-86
Daudi	Nanjing Cobioer Gene Technology	CAT#CBP60262
Cos7-hACE2	Beijing Vitalstar Biotechnology	CAT#hACE2-COS7
Huh7	Dr. Linqi Zhang	N/A
HeLa	Dr. Linqi Zhang	N/A
Oligonucleotides
siNT: UUCUCCGAACGUGUCACGUTT	this paper	N/A
siACE2-1: CCAUCUACAGUACUGGAAATT	this paper	N/A
siACE2-2: GGCCAUUAUAUGAAGAGUATT	this paper	N/A
CD16A forward: GACAGCGGCTCCTACTTCTG	this paper	N/A
CD16A reverse: AGTCCTGTGTCCACTGCAAA	this paper	N/A
CD32A forward: TCCCACAAGCAAACCACAGT	this paper	N/A
CD32A reverse: TGCTACAGCAGTCGCAATGA	this paper	N/A
CD32B forward: AGCGGATTTCAGCCAATCCC	this paper	N/A
CD32B reverse: ATACGGTTCTGGTCATCAGGC	this paper	N/A
CD64A forward: AAGTCACAATGGCACCTACC	this paper	N/A
CD64A reverse: GCTCAGGGTGACCAGATTCC	this paper	N/A
ACE2 forward: CATTGGTCTTCTGTCACCCGA	this paper	N/A
ACE2 reverse: ATGCGGGGTCACAGTATGTT	this paper	N/A
GAPDH forward: CTGCACCACCAACTGCTTAG	this paper	N/A
GAPDH reverse: GAGCTTCCCGTTCAGCTCAG	this paper	N/A
Recombinant DNA
pSARS-CoV-2-S, Wuhan-Hu-1 strain	this paper	N/A
pSARS-CoV-2-S, D614G	this paper	N/A
pSARS-CoV-2-S, N501Y-D614G	this paper	N/A
pSARS-CoV-2-S, E484K-N501Y-D614G	this paper	N/A
pSARS-CoV-2-S, B.1.351 strain	this paper	N/A
pMERS-CoV-S	Wang et al. (2020)	N/A
pMARV-GP	Dr. Linqi Zhang	N/A
pVSVG	Addgene	CAT#35616
pLP1	YouBio	CAT#VT1489
pLP2	YouBio	CAT#VT1490
pNL Luc E- R-	Wang et al. (2020)	N/A
pLenti-Cas9-Blast	Addgene	CAT#52962
pLenti-CD16A-Blast	this paper	N/A
pLenti-CD32A-Blast	this paper	N/A
pLenti-CD32B-Blast	this paper	N/A
pLenti-CD64A-Blast	this paper	N/A
pcDH-EF1-MCS-T2A-puro	SBI System Biosciences	CAT#CD527A-1
pcDH-FCER1G-puro	this paper	N/A
Software and algorithms
ImageStudio software	LI-COR Biosciences	https://www.licor.com/bio/image-studio/
GraphPad Prism software version 8.01	GraphPad Software, Inc.	https://www.graphpad.com/scientificsoftware/prism/
FlowJo V10	TreeStar	https://www.flowjo.com/