FIG. 1.

Agarose gel electrophoresis of amplified vanA, vanB, vanC1, vanC2, E. faecalis-specific, E. faecium-specific, and rrs genes by the optimized multiplex PCR assay containing seven primer sets. Lanes: M, 100-bp DNA ladder (New England Biolabs); 1, an E. faecalis vanA isolate; 2, an E. faecalis vanB isolate; 3, an E. faecium vanA isolate; 4, an E. faecium vanB isolate; 5, an E. gallinarum vanC1 isolate; 6, an E. casseliflavus vanC2 isolate; 7, a Pediococcus isolate; 8, a Leuconostoc isolate.