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. 2021 Dec 17;12:797854. doi: 10.3389/fpls.2021.797854

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Copyright © 2021 Feng, Yu, Lin, Yang, Chen, Liu, Sun, Zheng, Zhang and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CsCAT2-mediated theanine uptake into yeast cells. (A) Dot assays showed the growth phenotype of yeast lines 23344c (wild-type line), mutants 22Δ10α, 22Δ10α/pYES2 (pYES2), and 22Δ10α/pYES2-CsCAT2 (CsCAT2), on medium with 2 mM (NH₄)₂SO₄ or 2 mM theanine (Thea) as nitrogen source. (B) The growth curve of yeast in liquid medium at different theanine concentrations as nitrogen source. The OD₆₀₀ value was identified as a parameter for measuring yeast growth. 23344c and the empty vector pYES2 were the positive and negative controls, respectively. The result was expressed as mean ± SD (n = 3).