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. Author manuscript; available in PMC: 2022 Aug 19.
Published in final edited form as: Cell. 2021 Aug 2;184(17):4512–4530.e22. doi: 10.1016/j.cell.2021.07.015

Figure 6: DC3 trans-present IL-15, a critical survival signal for effector-like CTL in the TME.

Figure 6:

(A) Experimental protocol

(B) Selective depletion of cDC upon DT treatment of WT : zDCDTR −> WT mixed BMCs

(C, D) Ex vivo ZombieRed uptake (C) and ratios (D) of tumor-infiltrating WT and Cxcr6−/− TCF-1neg OT-I CTL

(E, F) Single-cell expression of Cd80 and Il15ra and (E) heatmap analysis of indicated transcripts in the D4M.3A-pOVA TME, ranked within indicated groups (F). See Table S1D for numerical data underlying the heatmap.

(G) Expression of indictated proteins by CCR7− cDC, YFP−, and -YFP+ DC3 in D4M.3A-pOVA tumors in IL-12 p40 YFP mice.

(H-K) Ex vivo uptake of ZombieRed (I), expression of Bim (J), and ratios (K) of WT and Cxcr6−/− TCF-1neg OT-I cells in D4M.3A-pOVA tumors in WT or Il15ra−/− hosts.

(L-O) Same as for (I-K), but following CTL injection into DT-treated Il15ra−/− : zDCDTR −> WT or WT : zDCDTR −> WT mixed BMCs

Graphs in B-D, G, I-K, and M-O show means and individual replicates. */**/***/**** = p<0.01/0.001/0.0001.